SangerAlignment Level

SangerContig Level

SangerRead Level

Basic Information:

  • Output Directory : /Users/camilogarcia/Documents/Teaching/BiologyCourses/BI0487/Lectures/biocomp-book/chs-challenges-demos/data/sanger-processed/rpoB/SangerAlignment/455_WT_rpoB/455_WT_rpoB_1_F
  • Input Directory : data/sanger-seqs/rpoB/.
  • Input Filename : 455_WT_rpoB_1_F.ab1
  • Read Feature : Forward Read

DNA Seqence

Primary Sequence

Secondary Sequence

Phred Quality Scores

Amino Acids Sequence

Amino Acids Sequence frameshit 0

Amino Acids Sequence frameshit 1

Amino Acids Sequence frameshit 2

Quality Trimming

  • Trimming Method: Method 1 ‘Logarithmic Scale Trimming’
  • M1 Trimming Cutoff: 1e-04
  • Raw Sequence Length: 1336
  • Trimmed Sequence Length: 889
  • Trimmed Start Point: 18
  • Trimmed End Point: 907
  • Raw Mean Quality Score: 43.3128743
  • Trimmed Mean Quality Score: 57.1406074
  • Raw Min Quality Score: 2
  • Trimmed Min Quality Score: 17
  • Remaining Read Ratio: 66.54 %

Chromatogram

  • Signal Ratio Cutoff: 0.33
  • Base Number Per Row: 100
  • Height Per Row: 200
  • Show Trimmed Region: TRUE 
## Chromatogram saved to /Users/camilogarcia/Documents/Teaching/BiologyCourses/BI0487/Lectures/biocomp-book/chs-challenges-demos/data/sanger-processed/rpoB/SangerAlignment/455_WT_rpoB/455_WT_rpoB_1_F/Chromatogram.pdf in the current working directory

Click Here to Open Chromatogram in New Page




Back to ‘SangerContig’ Report